Qt Designer when coupled with PyQt4 is usually used only for the layout process, as opposed to defining signals, buddies, etc. You perform the visual layout of your widgets, and save the .ui file.
Using pyuic4, you can then compile the .ui -> .py, and import that into your coded project.
Though there are probably 3 different approaches to using the UI at this point, what I typically do is multiple inheritance. If class MyMainWindow is meant to be a QMainWindow, then I inherit my class from QMainWindow, and the UI class.
Something like this...
pyuic4 myMainWindow.ui -o myMainWindowUI.py
main.py
from PyQt4 import QtGui
from myMainWindowUI import Ui_MainWindow
class MyMainWindow(QtGui.QMainWindow, Ui_MainWindow):
def __init__(self, *args, **kwargs)
super(MyMainWindow, self).__init__(*args, **kwargs)
self.setupUi(self)
The setupUi method applies your entire UI design to the class and you can now access all of the widgets you designed by their object names.
win = MyMainWindow()
print win.listWidget
print win.button1
win.show()