According to the docs you should be passing in the arguments as positional arguments, not as a tuple. If you want to use the values in a tuple, use the * operator.
str.format(*args, **kwargs)
Perform a string formatting operation. The string on which this method is called can contain literal text or replacement fields delimited by braces {}. Each replacement field contains either the numeric index of a positional argument, or the name of a keyword argument. Returns a copy of the string where each replacement field is replaced with the string value of the corresponding argument.
"The sum of 1 + 2 is {0}".format(1+2)
'The sum of 1 + 2 is 3'
More specifically, you'd want to do the following:
A = "{0}={1}"
B = ("str", "string")
C = A.format(*B)
print (C)
or
A = "{0}={1}"
C = A.format("str", "string")
print (C)