Node certainly supports binary data, that's kind of what Buffer is for. However, it is crucial to know what you are converting into what. For example, the emoji "☺️" is encoded as six bytes in UTF-8:
// UTF-16 (JS) string to UTF-8 representation
Buffer.from('☺️', 'utf-8')
// => <Buffer e2 98 ba ef b8 8f>
If you happen to have a string that is not a native JS string (i.e. its encoding is different), you can use the encoding parameter to make Buffer interpret each character in a different manner (though only several different conversions are supported). For example, if we have a string of six characters that correspond to the six numbers above, it is not a smiley face for JavaScript, but Buffer.from can help us repackage it:
Buffer.from('\u00e2\u0098\u00ba\u00ef\u00b8\u008f', 'binary')
// => <Buffer e2 98 ba ef b8 8f>
JavaScript itself has only one encoding for its strings; thus, the parameter 'binary' is not really the binary encoding, but a mode of operation for Buffer.from, telling it that the string would have been a binary string if each character were one byte (however, since JavaScript internally uses UCS-2, each character is always represented by two bytes). Thus, if you use it on something that is not a string of characters in range from U+0000 to U+00FF, it will not do the correct thing, because there no such thing (GIGO principle). What it will actually do is get the lower byte of each character, which is probably not what you want:
Buffer.from('STUFF', 'binary') // 8BIT range: U+0000 to U+00FF
// => <Buffer 42 59 54 45 53> ("STUFF")
Buffer.from('STUFF', 'binary') // U+FF33 U+FF34 U+FF35 U+FF26 U+FF26
// => <Buffer 33 34 35 26 26> (garbage)
So, Node's Buffer structure exactly corresponds to Ruby's ASCII-8BIT "encoding" (binary is an encoding like "bald" is a hair style — it simply means no interpretation is attached to bytes; e.g. in ASCII, 65 means "A"; but in binary "encoding", 65 is just 65). Buffer.from with 'binary' lets you convert weird strings where one character corresponds to one byte into a Buffer. It is not the normal way of handling binary data; its function is to un-mess-up binary data when it has been read incorrectly into a string.
I assume you are reading a file as string, then trying to convert it to a Buffer — but your string is not actually in what Node considers to be the "binary" form (a sequence of characters in range from U+0000 to U+00FF; thus "in Node.js binary maps to ISO-8859-1" is not really true, because ISO-8859-1 is a sequence of characters in range from 0x00 to 0xFF — a single-byte encoding!).
Ideally, to have a binary representation of file contents, you would want to read the file as a Buffer in the first place (by using fs.readFile without an encoding), without ever touching a string.
(If my guess here is incorrect, please specify what the contents of your < text input > is, and how you obtain it, and in which case "it doesn't result in the same amount of bytes".)
EDIT: I seem to like typing Array.from too much. It's Buffer.from, of course.