I'm using the PYRFC library and so far I've managed to connect to SAP.
conn = Connection(ashost='xxxxxxxxx', sysnr='02', client='100', user='xxxxx', passwd='xxxxxxxx.')
result = conn.call('STFC_CONNECTION', REQUTEXT=u'Hello SAP!')
print (result)
I did the connection test and everything was ok. But now I'm trying to run the queues created in SAP.
I performed some tests, trying to simulate the F8 but without success. Is there any way to make this execution using via python?

trying to simulate the F8 but without successwhat success do you expect? you are trying to run SMQ1/SMQ2 functionality from PyRFC?